Determination of carotenes by thin-layer chromatography.

The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel plates F254 and the solvent system of mobile phase acetone-water 9:1. In the tomato, βcarotene was identified and was caltulate Rf of β-carotene.